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Evaluation of Sigatoka leaf spot (Mycosphaerella musicola Leach)

disease resistance in banana using morphological and molecular markers
door Nwauzoma Akagbuo Barth - Verkocht door Dodax EU
Staat: Nieuw
€ 48,81
Incl. BTW - Gratis verzenden
Nwauzoma Akagbuo Barth Evaluation of Sigatoka leaf spot (Mycosphaerella musicola Leach)
Nwauzoma Akagbuo Barth - Evaluation of Sigatoka leaf spot (Mycosphaerella musicola Leach)

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Levering: tussen 2020-12-10 en 2020-12-14
Verkoop en verzending: Dodax EU

Beschrijving

Sigatoka leaf spot disease limits banana production. Breeding for resistance is the most effective method to control Musa diseases. However, Musa improvement using conventional methods is difficult due to lack of genetic variability, hence biotechnology. Marker-assisted selection is a reliable method to improve disease resistance in Musa. This study was to identify markers that may be linked to Sigatoka leaf spot disease in Musa, using RAPDs and converting such into SCAR. A total of 102 oligonucleotide OPERON primers were used to screen genomic DNA from resistant and susceptible cultivars with only 10.8% polymorphism. Eventually, OPK01 and OPK11 primers in Calcutta 4 were eluted, but only OPK 11 was sequenced and cloned using pGEM-2T vector, resulting to a band size of 4.3 KB, and the development of two SCAR markers. A FASTA search in the Musa database could not identify homologous gene sequences to the PCR fragment. The SCAR marker was used to amplify genomic DNA from the segregating population which could not discriminate between resistant and susceptible samples due to amplification conditions, limited number of primers and absence of tight linkage with the gene of interest.

Medewerkers

Auteur Nwauzoma Akagbuo Barth

Productdetails

DUIN 13SHV1LD5HQ

GTIN 9783844358155

Taal Engels

Pagina-aantallen 84

Product type Paperback

Maat 220  mm

€ 48,81
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