Applications of Mass Spectrometry in Microbiology - From Strain Characterization to Rapid Screening for Antibiotic Resistance
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While previous work has covered broader approaches in using MS to characterize microorganisms at the species level or above, this book focuses on strain-level and subtyping applications. In twelve individual chapters, innovators, leaders and practitioners in the field from around the world have contributed to a comprehensive overview of current and next-generation approaches for MS-based microbial characterization at the subspecies and strain levels. Chapters include up-to-date reference lists as well as web-links to databases, recommended software, and other useful tools.
The emergence of new, antibiotic-resistant strains of human or animal pathogens is of extraordinary concern not only to the scientific and medical communities, but to the general public as well. Developments of novel MS-based assays for rapid identification of strains of antibiotic-resistant microorganisms are reviewed in the book as well. Microbiologists, bioanalytical scientists, infectious disease specialists, clinical laboratory and public health practitioners as well as researchers in universities, hospitals, government labs, and the pharmaceutical and biotechnology industries will find this book to be a timely and valuable resource.
Introduction.- SECTION I. METHODOLOGY AND TECHNIQUES.- Methods and instrumentation in mass spectrometry for the differentiation of closely related microorganisms.- Sample preparation methods for the rapid MS analysis of microorganisms.- Advantages offered by proteomic strategies for rapid biodetection.- Bottom Up Proteomics Methods for Strain-Level Typing and Identification of Bacteria.- Maximizing the taxonomic resolution of MALDI-TOF-MS-based approaches to bacterial characterization: From culture conditions through data analysis.- SECTION II. SUB-SPECIES DISCRIMINATION.-Modulation of the Discriminatory Power of MALDI-TOF MS Profiling for Distinguishing between Closely Related Bacterial Strains.- Discriminatory Power of MALDI-TOF Mass Spectrometry for Phylogenetically Closely Related Microbial Strains.- MALDI-TOF MS as a novel tool for dereplication and characterization of microbiota in bacterial diversity studies.- Bacterial Identification at the Serovar level by Top-down Mass Spectrometry.- SECTION III. DRUG RESISTANCE MONITORING AND ASSAYS.- Rapid profiling of human pathogenic bacteria and antibiotic resistance employing specific tryptic peptides as biomarkers.- Detection of β-lactamases and their activity using MALDI-TOF MS.- Stable-isotope based strategies for rapid determination of drug resistance by mass spectrometry.
The applications have wide appeal, from practitioners to public health professionals
Contributions from a broad range of authors, encompassing all aspects of the field, will lend the volume a balanced and encyclopedic perspective?
Includes supplementary material: sn.pub/extras
Todd Sandrin, School of Mathematical and Natural Sciences, Arizona State University