Receptor Signal Transduction Protocols - Third Edition

84 schwarz-weiße Abbildungen, 6 schwarz-weiße Tabellen

Part I: Developing Models for Studying GPCRs 1. Inducible Expression of G Protein-Coupled Receptors in Transfected Cells            Beryl Koener and Emmanuel Hermans 2. Using the Flp-InTM T-RexTM System to Regulate GPCR Expression            Richard J. Ward, Elisa Alvarez-Curto, and Graeme Milligan 3. Viral Infection for GPCR Expression in Eukaryotic Cells            Antonio Porcellini, Luisa Iacovelli, and Antonio De Blasi 4. Generation of Epitope-Tagged GPCRs            Yan Huang and Gary B. Willars 5. The Use of Site-Directed Mutagenesis to Study GPCRs            Alex C. Conner, James Barwell, David R. Poyner, and Mark Wheatley 6. Approaches to Study GPCR Regulation in Native Systems            Jonathon M. Willets 7. Heterologous Expression of GPCRs in Fission Yeast            John Davey and Graham Ladds Part II: Examining GPCR Expression and Agonist-Induced Covalent Modifications 8. Radioligand Binding Methods for Membrane Preparations and Intact Cells            David B. Bylund and Myron L. Toews 9. Quantification of GPCR mRNA Using Real-Time RT-PCR            Trond Brattelid and Finn Olav Levy 10. Determining Allosteric Modulator Mechanism of Action: Integration of Radioligand Binding and Functional Assay Data            Christopher J. Langmead 11. Design and Use of Fluorescent Ligands to Study Ligand-Receptor Interactions in Single Living Cells            Stephen J. Briddon, Barrie Kellam, and Stephen J. Hill 12. Examining Site-Specific GPCR Phosphorylation            Adrian J. Butcher, Andrew B. Tobin, and Kok Choi Kong 13. Ubiquitination of GPCRs            Adriana Caballero and Adriano Marchese Part III: Examining Early Events in GPCR Signaling 14. [35S]GTPγS Binding as an Index of Total G-Protein and Gα-Subtype-Specific Activation by GPCRs            Rajendra Mistry, Mark R. Dowling, and R.A. John Challiss 15. Using Calcium Imaging as a Readout of GPCR Activation            Martin D. Bootman and H. Llewelyn Roderick 16. Measuring Spatio-Temporal Dynamics of Cyclic AMP Signaling in Real-Time Using FRET-Based Biosensors            Frank Gesellchen, Alessandra Stangherlin, Nicoletta Surdo, Anna Terrin, Anna Zoccarato, and Manuela Zaccolo 17. Determining the Activation of Rho as an Index of Receptor Coupling to G12/13 Proteins            Michio Nakaya, Mina Ohba, Motohiro Nishida, and Hitoshi Kurose 18. The Use of Translocating Fluorescent Biosensors for Real-Time Monitoring of GPCR-Mediated Signaling Events            Carl P. Nelson and R.A. John Challiss Part IV: Receptor-Receptor and Receptor-Protein Interactions 19. Study of GPCR-Protein Interactions Using Gel Overlay Assays and Glutathione-S-Transferase-Fusion Protein Pull-Downs            Ashley E. Brady, Yunjia Chen, Lee E. Limbird, and Qin Wang 20. Study of GPCR-Protein Interactions by BRET            Martina Kocan and Kevin D.G. Pfleger 21. Time Resolved FRET Strategy with Fluorescent Ligands to Analyze Receptor Interactions in Native Tissues: Application to GPCR Oligomerization            Martin Cottet, Laura Albizu, Laetitia Comps-Agrar, Eric Trinquet, Jean-Philippe Pin, Bernard Mouillac, and Thierry Durroux 22. Peptide Affinity Purification for the Isolation and Identification of GPCR-Associated Protein Complexes            Pascal Maurice, Avais M. Daulat, and Ralf Jockers 23. Tandem Affinity Purification and Identification of GPCR-Associated Protein Complexes            Avais M. Daulat, Pascal Maurice, and Ralf Jockers 24. Identification of GPCR Localization in Detergent Resistant Membranes            Ranju Kumari and Anna Francesconi 25. Analysis of GPCR Localization and Trafficking            James N. Hislop and Mark von Zastrow Part V: Statistical Methods 26. Statistical Methods in Research            Domenico Spina

Willars, Gary B.;Willars

Challiss, R.A. John;Challiss

Acknowledges the constant evolution of the GPCR field with up-to-date methodologies

Contains detailed revisions of chapters from the prior editions as well as a considerable amount of completely new material

Features expert tips and key implementation advice

Includes supplementary material: sn.pub/extras

Hardcover

Humana Press

From the reviews of the third edition:

"This is an extensive collection of established, new, and updated techniques for the study of the consequences of receptor activation. Each of the 26 chapters offers a specific recipe, with lists of required reagents and equipment, plus notes to aid in use of the protocol. ... This book would be most useful to researchers already familiar with fundamental molecular biological or biochemical laboratory techniques." (Kenneth E. McCarson, Doody's Book Reviews, February, 2012)

Engels

3rd ed. 2011

479

In this third edition of Receptor Signal Transduction Protocols, the expert contributors take into account the constant evolution of the GPCR field. It contains detailed revisions of chapters from the prior editions and a considerable amount of new material.